Influence of cytomegalovirus infection on nucleoside diphosphatase activity in placenta

Introduction . Cytomegalovirus infection (CMVI) makes a significant contribution to the development of pregnancy pathologies, including dysfunction placenta. However, mechanism such an effect CMV has not been precisely established. For coordination biochemical reactions in placenta, optimal concentration substances, nucleotides, is required. Nucleoside diphosphatase enzyme that catalyzes hydrolysis nucleotide diphosphates and phosphate. Aim Determination activity nucleoside placenta physiological complicated CMVI reactivation third trimester pregnancy. Materials methods A study 62 placentas obtained during childbirth at 38-40 weeks was carried out. The first group consisted from women with second – without history infectious pathology. Diagnosis out by determining antibodies class M G ELISA, as well DNA detected PCR. Histochemical analysis performed according method A.B.Novikoff D.S.Goldfischer modified Z.Lojda et al. Results In group, nucleoside-diphosphatase had pronounced syncytiotrophoblast connective tissue stroma villi. When reactivated pregnancy, decrease intensity histochemical reaction found: cytophotometric index significantly (p<0.001) decreased 13.06±0.089 rel. units (in it 14.11±0.119 relative units). Conclusion found might indicate metabolism our opinion, enzymes involved exchange nucleotides will help reveal mechanisms which causes disturbances functioning leading complications